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KMID : 0364820120480040270
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Korean Journal of Microbiology
2012 Volume.48 No. 4 p.270 ~ p.274
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Isolation and Characterization of Streptomyces spp from Soil Showing Broad Spectrum Antibiotic Activity
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Park Se-Wook
Bae Tae-Ok
Kim Seung-Bum
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Abstract
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Three actinobacterial strains exhibiting broad spectrum antibiotic activities were isolated from soil, and
characterized. Through the comparative analysis of 16S rRNA genes, the three isolates could be assigned to the
genus Streptomyces, as S. tanashiensis, S. nashivillensis, and S. rubiginosohelvolus were found to be the mostly
related species, but the strains formed independent phylogenetic lineage. Each strain exhibited different
antimicrobial profile against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus, Gram-negative
bacteria Salmonella typhi, Enterobacter cloacae, Serratia marcescens, and Pseudomonas aeruginosa, and also fungi
Candida tropicalis and Candida krusei. In addition to the antimicrobial profile, the strains also differed in API ZYM
test results, which implies that the three strains might produce difference antimicrobial substances.
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KEYWORD
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Streptomyces, antimicrobial activity
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